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clear bottomplate  (CELLTREAT Scientific)


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    Structured Review

    CELLTREAT Scientific clear bottomplate
    Clear Bottomplate, supplied by CELLTREAT Scientific, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/clear+bottomplate/Non-treated+Multiple+Well+Plates/pm40595444-205-14-16
    Average 94 stars, based on 2 article reviews
    clear bottomplate - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Membrane:

    Article Title: Gut microbial utilization of the alternative sweetener, D-allulose, via AlsE.
    Article Snippet: 25 μL of the overnight culture was inoculated in 2mL triplicates ofminimalmedia (M9) supplementedwith 100 μM Isopropyl β- d1-thiogalactopyranoside (IPTG, GoldBio, I2481C25), 100 μg/mL carbenicillin, 50 μg/mL kanamycin, 10mg/mL Glucose (Sigma-Aldrich, 310042), 5mg/mLAllose (Chem-Impex, 27965), and 10mg/mLD-allulose12 (ChemImpex, 32353). .. For kinetic measurements, 250 μL of each triplicate was aliquoted into a 96-well acrylic, clear bottomplate (Celltreat, 229592), sealed with a Breathe Easy membrane (Electron Microscopy Sciences, 70536-10), and incubated at 37 °C for 48–70 h, depending on the strain observed. .. The average absorbance at 600 nm was measured with a Spectramax M5 plate reader. pCW-lic_C.inn_alsE & pCW-lic-E.coli_alsE constructs In order to achieve ectopic expression of alsE from Clostridium innocuum 6_1_30 and E. coli JW276035 in the knockout mutants, the alsE gene was amplified and cloned into the pCW-lic vector backbone (Addgene plasmid # 26098; http://n2t.net/addgene:26098; RRID:Addgene_26098).

    Electron Microscopy:

    Article Title: Gut microbial utilization of the alternative sweetener, D-allulose, via AlsE.
    Article Snippet: 25 μL of the overnight culture was inoculated in 2mL triplicates ofminimalmedia (M9) supplementedwith 100 μM Isopropyl β- d1-thiogalactopyranoside (IPTG, GoldBio, I2481C25), 100 μg/mL carbenicillin, 50 μg/mL kanamycin, 10mg/mL Glucose (Sigma-Aldrich, 310042), 5mg/mLAllose (Chem-Impex, 27965), and 10mg/mLD-allulose12 (ChemImpex, 32353). .. For kinetic measurements, 250 μL of each triplicate was aliquoted into a 96-well acrylic, clear bottomplate (Celltreat, 229592), sealed with a Breathe Easy membrane (Electron Microscopy Sciences, 70536-10), and incubated at 37 °C for 48–70 h, depending on the strain observed. .. The average absorbance at 600 nm was measured with a Spectramax M5 plate reader. pCW-lic_C.inn_alsE & pCW-lic-E.coli_alsE constructs In order to achieve ectopic expression of alsE from Clostridium innocuum 6_1_30 and E. coli JW276035 in the knockout mutants, the alsE gene was amplified and cloned into the pCW-lic vector backbone (Addgene plasmid # 26098; http://n2t.net/addgene:26098; RRID:Addgene_26098).

    Incubation:

    Article Title: Gut microbial utilization of the alternative sweetener, D-allulose, via AlsE.
    Article Snippet: 25 μL of the overnight culture was inoculated in 2mL triplicates ofminimalmedia (M9) supplementedwith 100 μM Isopropyl β- d1-thiogalactopyranoside (IPTG, GoldBio, I2481C25), 100 μg/mL carbenicillin, 50 μg/mL kanamycin, 10mg/mL Glucose (Sigma-Aldrich, 310042), 5mg/mLAllose (Chem-Impex, 27965), and 10mg/mLD-allulose12 (ChemImpex, 32353). .. For kinetic measurements, 250 μL of each triplicate was aliquoted into a 96-well acrylic, clear bottomplate (Celltreat, 229592), sealed with a Breathe Easy membrane (Electron Microscopy Sciences, 70536-10), and incubated at 37 °C for 48–70 h, depending on the strain observed. .. The average absorbance at 600 nm was measured with a Spectramax M5 plate reader. pCW-lic_C.inn_alsE & pCW-lic-E.coli_alsE constructs In order to achieve ectopic expression of alsE from Clostridium innocuum 6_1_30 and E. coli JW276035 in the knockout mutants, the alsE gene was amplified and cloned into the pCW-lic vector backbone (Addgene plasmid # 26098; http://n2t.net/addgene:26098; RRID:Addgene_26098).



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